CHFR monoclonal antibody (M01), clone 1H3-A12
产品名称: CHFR monoclonal antibody (M01), clone 1H3-A12
英文名称: CHFR monoclonal antibody (M01), clone 1H3-A12
产品编号: H00055743-M01
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant CHFR.
- Immunogen:
- CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (97.46 KDa) .
- MSDS:
- Download
- Publication Reference
- 1.
- CHFR regulates the mitotic checkpoint by targeting PARP-1 for ubiquitination and degradation.
Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T.J Biol Chem. 2012 Feb 15. [Epub ahead of print]
- 2.
- Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach.
Fujita A, Yamamoto H, Imamura M, Nakamura N, Maehara Y, Tsuneyoshi M, Oda Y.Virchows Arch. 2011 Dec 22.
- 3.
- Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer.
Hu SL, Huang DB, Sun YB, Wu L, Xu WP, Yin S, Chen J, Jiang XD, Shen G.Med Oncol. 2011 Jun;28(2):447-54. Epub 2010 Mar 19.
- 4.
- Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.
Gao YJ, Xin Y, Zhang JJ, Zhou J.World J Gastroenterol. 2008 Aug 28;14(32):5000-7.
- 5.
- Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.
Privette LM, Weier JF, Nguyen HN, Yu X, Petty EM.Neoplasia. 2008 Jul;10(7):643-52.
- 6.
- Altered Expression of the Early Mitotic Checkpoint Protein, CHFR, in Breast Cancers: Implications for Tumor Suppression.
Privette LM, Gonzalez ME, Ding L, Kleer CG, Petty EM.Cancer Res. 2007 Jul 1;67(13):6064-74. Epub 2007 Jun 27.
- 7.
- Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.
Milne AN, Sitarz R, Carvalho R, Polak MM, Ligtenberg M, Pauwels P, Offerhaus GJ, Weterman MA.Hum Pathol. 2007 Jun;38(6):903-13. Epub 2007 Mar 21.
- Applications
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge this image
- Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 55743
- GeneBank Accession#:
- BC012072
- Protein Accession#:
- AAH12072.1
- Gene Name:
- CHFR
- Gene Alias:
- FLJ10796,FLJ33629,RNF116,RNF196
- Gene Description:
- checkpoint with forkhead and ring finger domains
- Omim ID:
- 605209
- Gene Ontology:
- Hyperlink
- Other Designations:
- -
- Related Disease
- Carcinoma, Squamous Cell
- Colorectal Neoplasms
- Genetic Predisposition to Disease
- Head and Neck Neoplasms
- Microsatellite Instability
- Neoplasm Metastasis
- Neoplasms, Second Primary
- Obesity
- Ovarian Failure, Premature
- Polycystic Ovary Syndrome
- Puberty, Delayed
- Puberty, Precocious
- Recurrence
- Thrombophilia
- Tobacco Use Disorder