MAP3K14 (Human) Recombinant Protein
产品名称： MAP3K14 (Human) Recombinant Protein
英文名称： MAP3K14 (Human) Recombinant Protein
产品编号： P5725
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Human MAP3K14 (NP_003945.1, 658 a.a. - 1114 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 96
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf45) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 76 % by SDS-PAGE/CBB staining.
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).
Substrate : BTN-IKKa [inactive mutant]. ATP: 100 µM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 9020
- Protein Accession#:
- NP_003945.1
- Gene Name:
- MAP3K14
- Gene Alias:
- FTDCR1B,HS,HSNIK,NIK
- Gene Description:
- mitogen-activated protein kinase kinase kinase 14
- Omim ID:
- 604655
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq
- Other Designations:
- serine/threonine protein-kinase
- Related Disease